Q15046

PTMD Annotation Information

※ Protein Information

Tag	Content
UniProt Accession	SYK_HUMAN; Q15046;
Entrez ID	3735
GenBank Protein ID	NM_001130089.1; NM_005548.2;
GenBank Nucleotide ID	NP_001123561.1; NP_005539.1;
Protein Name	Lysine--tRNA ligase (EC 2.7.7.-) (EC 6.1.1.6) (Lysyl-tRNA synthetase) (LysRS)
Gene Name	KARS; KIAA0070
Organism	Homo sapiens
NCBI Taxa ID	9606
Functional Description	Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739).;(Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. Functional Description Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739).;(Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
Sequence (Fasta)	MAAVQAAEVK VDGSEPKLSK NELKRRLKAE KKVAEKEAKQ KELSEKQLSQ ATAAATNHTT 60 DNGVGPEEES VDPNQYYKIR SQAIHQLKVN GEDPYPHKFH VDISLTDFIQ KYSHLQPGDH 120 LTDITLKVAG RIHAKRASGG KLIFYDLRGE GVKLQVMANS RNYKSEEEFI HINNKLRRGD 180 IIGVQGNPGK TKKGELSIIP YEITLLSPCL HMLPHLHFGL KDKETRYRQR YLDLILNDFV 240 RQKFIIRSKI ITYIRSFLDE LGFLEIETPM MNIIPGGAVA KPFITYHNEL DMNLYMRIAP 300 ELYHKMLVVG GIDRVYEIGR QFRNEGIDLT HNPEFTTCEF YMAYADYHDL MEITEKMVSG 360 MVKHITGSYK VTYHPDGPEG QAYDVDFTPP FRRINMVEEL EKALGMKLPE TNLFETEETR 420 KILDDICVAK AVECPPPRTT ARLLDKLVGE FLEVTCINPT FICDHPQIMS PLAKWHRSKE 480 GLTERFELFV MKKEICNAYT ELNDPMRQRQ LFEEQAKAKA AGDDEAMFID ENFCTALEYG 540 LPPTAGWGMG IDRVAMFLTD SNNIKEVLLF PAMKPEDKKE NVATTDTLES TTVGTSV 598 Fasta Sequence >Q15046\|KARS; KIAA0070\|Homo sapiens (Human) MAAVQAAEVKVDGSEPKLSKNELKRRLKAEKKVAEKEAKQKELSEKQLSQATAAATNHTTDNGVGPEEESVDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV

※ PTM-Disease Association

Num	PTM	Disease	Cell Type	Type	PTM Site	PMID
1	Serine Phosphorylation	Melanoma		U	S207	28394346
1	[Reference]: Ap4A is mainly produced in the mammalian cells by S207-phosphorylated Lysyl-tRNA synthetase.?Mutational analysis confirmed that these modifications promote MITF transcriptional and oncogenic activity in melanoma cell lines, derived from human melanoma patients.

※ Disease Cross-ref Annotation

Database	Annotation
CTD (Curated) (count: 2)	OMIM:613641 ; CHARCOT-MARIE-TOOTH DISEASE, RECESSIVE INTERMEDIATE B OMIM:613916 ; DEAFNESS, AUTOSOMAL RECESSIVE 89
DisGeNet (Curated) (count: 20) (view all)	C0011053; Deafness C0018772; Hearing Loss, Partial C0027859; Acoustic Neuroma C0085684; Foot-drop C0234146; Absent reflex C0241772; Reflex, Deep Tendon, Absent C0278124; Absent tendon reflex C0339789; Congenital deafness C0427149; Gait, Drop Foot C0557874; Global developmental delay C0700078; Decreased tendon reflex C0728829; Congenital pes cavus C1384666; hearing impairment C1847584; Distal sensory impairment C1864897; Cognitive delay C1866141; Foot dorsiflexor weakness C3150897; CHARCOT-MARIE-TOOTH DISEASE, RECESSIVE INTERMEDIATE B C3151351; DEAFNESS, AUTOSOMAL RECESSIVE 89 C4020875; Mental and motor retardation C4020899; Autosomal recessive predisposition
GWASdb (count: 1)	rs3784929; Alzheimer's disease; Alzheimer's disease

※ PTM Sites

PTM	Modification Sites
Phosphorylation (count: 10) (view all)	207 PYEITLLSPCLHMLP dbPAF 366 SGMVKHITGSYKVTY dbPAF 470 CDHPQIMSPLAKWHR dbPAF 49 ELSEKQLSQATAAAT dbPAF 52 EKQLSQATAAATNHT dbPAF 561 VAMFLTDSNNIKEVL dbPAF 587 ENVATTDTLESTTVG dbPAF 590 ATTDTLESTTVGTSV dbPAF 596 ESTTVGTSV****** dbPAF 81 NQYYKIRSQAIHQLK dbPAF
Acetylation (count: 15) (view all)	111 SLTDFIQKYSHLQPG PLMD 127 HLTDITLKVAGRIHA PLMD 141 AKRASGGKLIFYDLR PLMD 153 DLRGEGVKLQVMANS PLMD 164 MANSRNYKSEEEFIH PLMD 175 EFIHINNKLRRGDII PLMD 363 KMVSGMVKHITGSYK PLMD 402 NMVEELEKALGMKLP PLMD 430 LDDICVAKAVECPPP PLMD 492 RFELFVMKKEICNAY PLMD 493 FELFVMKKEICNAYT PLMD 517 QLFEEQAKAKAAGDD PLMD 574 VLLFPAMKPEDKKEN PLMD 78 VDPNQYYKIRSQAIH PLMD 88 SQAIHQLKVNGEDPY PLMD
Ubiquitination (count: 24) (view all)	10 AVQAAEVKVDGSEPK PLMD 127 HLTDITLKVAGRIHA PLMD 141 AKRASGGKLIFYDLR PLMD 164 MANSRNYKSEEEFIH PLMD 17 KVDGSEPKLSKNELK PLMD 175 EFIHINNKLRRGDII PLMD 190 GVQGNPGKTKKGELS PLMD 192 QGNPGKTKKGELSII PLMD 249 QKFIIRSKIITYIRS PLMD 305 IAPELYHKMLVVGGI PLMD 363 KMVSGMVKHITGSYK PLMD 402 NMVEELEKALGMKLP PLMD 407 LEKALGMKLPETNLF PLMD 421 FETEETRKILDDICV PLMD 430 LDDICVAKAVECPPP PLMD 474 QIMSPLAKWHRSKEG PLMD 492 RFELFVMKKEICNAY PLMD 493 FELFVMKKEICNAYT PLMD 517 QLFEEQAKAKAAGDD PLMD 565 LTDSNNIKEVLLFPA PLMD 574 VLLFPAMKPEDKKEN PLMD 578 PAMKPEDKKENVATT PLMD 579 AMKPEDKKENVATTD PLMD 78 VDPNQYYKIRSQAIH PLMD
Sumoylation (count: 1)	10 AVQAAEVKVDGSEPK PLMD
Malonylation (count: 1)	141 AKRASGGKLIFYDLR PLMD
Succinylation (count: 1)	141 AKRASGGKLIFYDLR PLMD

※ Protein-Protein Interaction

Network

Interaction

A

B

Source

P00441	Q15046	HPRD
Q14164	Q15046	IntAct