Q07912

※ Protein Information

Tag	Content
UniProt Accession	ACK1_HUMAN; Q07912;
Entrez ID	10188
GenBank Protein ID	NM_001010938.1; NM_001308046.1; NM_005781.4;
GenBank Nucleotide ID	NP_001010938.1; NP_001294975.1; NP_005772.3;
Protein Name	Activated CDC42 kinase 1 (ACK-1) (EC 2.7.10.2) (EC 2.7.11.1) (Tyrosine kinase non-receptor protein 2)
Gene Name	TNK2; ACK1
Organism	Homo sapiens
NCBI Taxa ID	9606
Functional Description	Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR (view all) Functional Description Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP (PubMed:20110370).
Sequence (Fasta)	MQPEEGTGWL LELLSEVQLQ QYFLRLRDDL NVTRLSHFEY VKNEDLEKIG MGRPGQRRLW 60 EAVKRRKALC KRKSWMSKVF SGKRLEAEFP PHHSQSTFRK TSPAPGGPAG EGPLQSLTCL 120 IGEKDLRLLE KLGDGSFGVV RRGEWDAPSG KTVSVAVKCL KPDVLSQPEA MDDFIREVNA 180 MHSLDHRNLI RLYGVVLTPP MKMVTELAPL GSLLDRLRKH QGHFLLGTLS RYAVQVAEGM 240 GYLESKRFIH RDLAARNLLL ATRDLVKIGD FGLMRALPQN DDHYVMQEHR KVPFAWCAPE 300 SLKTRTFSHA SDTWMFGVTL WEMFTYGQEP WIGLNGSQIL HKIDKEGERL PRPEDCPQDI 360 YNVMVQCWAH KPEDRPTFVA LRDFLLEAQP TDMRALQDFE EPDKLHIQMN DVITVIEGRA 420 ENYWWRGQNT RTLCVGPFPR NVVTSVAGLS AQDISQPLQN SFIHTGHGDS DPRHCWGFPD 480 RIDELYLGNP MDPPDLLSVE LSTSRPPQHL GGVKKPTYDP VSEDQDPLSS DFKRLGLRKP 540 GLPRGLWLAK PSARVPGTKA SRGSGAEVTL IDFGEEPVVP ALRPCAPSLA QLAMDACSLL 600 DETPPQSPTR ALPRPLHPTP VVDWDARPLP PPPAYDDVAQ DEDDFEICSI NSTLVGAGVP 660 AGPSQGQTNY AFVPEQARPP PPLEDNLFLP PQGGGKPPSS AQTAEIFQAL QQECMRQLQA 720 PAGSPAPSPS PGGDDKPQVP PRVPIPPRPT RPHVQLSPAP PGEEETSQWP GPASPPRVPP 780 REPLSPQGSR TPSPLVPPGS SPLPPRLSSS PGKTMPTTQS FASDPKYATP QVIQAPGPRA 840 GPCILPIVRD GKKVSSTHYY LLPERPSYLE RYQRFLREAQ SPEEPTPLPV PLLLPPPSTP 900 APAAPTATVR PMPQAALDPK ANFSTNNSNP GARPPPPRAT ARLPQRGCPG DGPEAGRPAD 960 KIQMAMVHGV TTEECQAALQ CHGWSVQRAA QYLKVEQLFG LGLRPRGECH KVLEMFDWNL 1020 EQAGCHLLGS WGPAHHKR 1039 Fasta Sequence >Q07912\|TNK2; ACK1\|Homo sapiens (Human) MQPEEGTGWLLELLSEVQLQQYFLRLRDDLNVTRLSHFEYVKNEDLEKIGMGRPGQRRLWEAVKRRKALCKRKSWMSKVFSGKRLEAEFPPHHSQSTFRKTSPAPGGPAGEGPLQSLTCLIGEKDLRLLEKLGDGSFGVVRRGEWDAPSGKTVSVAVKCLKPDVLSQPEAMDDFIREVNAMHSLDHRNLIRLYGVVLTPPMKMVTELAPLGSLLDRLRKHQGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRALPQNDDHYVMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGLNGSQILHKIDKEGERLPRPEDCPQDIYNVMVQCWAHKPEDRPTFVALRDFLLEAQPTDMRALQDFEEPDKLHIQMNDVITVIEGRAENYWWRGQNTRTLCVGPFPRNVVTSVAGLSAQDISQPLQNSFIHTGHGDSDPRHCWGFPDRIDELYLGNPMDPPDLLSVELSTSRPPQHLGGVKKPTYDPVSEDQDPLSSDFKRLGLRKPGLPRGLWLAKPSARVPGTKASRGSGAEVTLIDFGEEPVVPALRPCAPSLAQLAMDACSLLDETPPQSPTRALPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDFEICSINSTLVGAGVPAGPSQGQTNYAFVPEQARPPPPLEDNLFLPPQGGGKPPSSAQTAEIFQALQQECMRQLQAPAGSPAPSPSPGGDDKPQVPPRVPIPPRPTRPHVQLSPAPPGEEETSQWPGPASPPRVPPREPLSPQGSRTPSPLVPPGSSPLPPRLSSSPGKTMPTTQSFASDPKYATPQVIQAPGPRAGPCILPIVRDGKKVSSTHYYLLPERPSYLERYQRFLREAQSPEEPTPLPVPLLLPPPSTPAPAAPTATVRPMPQAALDPKANFSTNNSNPGARPPPPRATARLPQRGCPGDGPEAGRPADKIQMAMVHGVTTEECQAALQCHGWSVQRAAQYLKVEQLFGLGLRPRGECHKVLEMFDWNLEQAGCHLLGSWGPAHHKR

※ PTM-Disease Association

Num	PTM	Disease	Type	PTM Site	PMID
1	Tyrosine Phosphorylation	Breast cancer/tumor/carcinoma	U	Y284	20333297
1	[Reference]: Ack1 Tyr284 phosphorylation is prognostic of progression of prostate cancer and inhibitors of Ack1 activity could be novel therapeutic agents to treat AIPC
2	Tyrosine Phosphorylation	Prostate cancer/carcinoma/adenocarcinoma	U	Y284	20623637
2	[Reference]: Ack1 Tyr284 phosphorylation is prognostic of progression of prostate cancer and inhibitors of Ack1 activity could be novel therapeutic agents to treat AIPC
3	Tyrosine Phosphorylation	Glioblastoma	N	Y635	25257795
3	[Reference]: Glioma cell cycle progression, proliferation and tumorigenesis were accordingly blocked by ACK1 Y635F or T325A.?

※ Disease Cross-ref Annotation

Database	Annotation
CTD (Curated) (count: 2)	MESH:D008113 ; Liver Neoplasms MESH:D011230 ; Precancerous Conditions
DisGeNet (Curated) (count: 2)	C0023903; Liver neoplasms C0032927; Precancerous Conditions
GWASdb (count: 2)	rs112384084; Prostate cancer; prostate cancer rs2278034; Bronchopulmonary dysplasia; bronchopulmonary dysplasia

※ PTM Sites

PTM	Modification Sites
Phosphorylation (count: 39) (view all)	101 SQSTFRKTSPAPGGP dbPAF 102 QSTFRKTSPAPGGPA dbPAF 136 LEKLGDGSFGVVRRG dbPAF 149 RGEWDAPSGKTVSVA dbPAF 193 HRNLIRLYGVVLTPP dbPAF 205 TPPMKMVTELAPLGS dbPAF 212 TELAPLGSLLDRLRK dbPAF 284 LPQNDDHYVMQEHRK dbPAF 36 DLNVTRLSHFEYVKN dbPAF 465 LQNSFIHTGHGDSDP dbPAF 517 LGGVKKPTYDPVSED dbPAF 518 GGVKKPTYDPVSEDQ dbPAF 529 SEDQDPLSSDFKRLG dbPAF 603 ACSLLDETPPQSPTR dbPAF 607 LDETPPQSPTRALPR dbPAF 635 PLPPPPAYDDVAQDE dbPAF 724 QLQAPAGSPAPSPSP dbPAF 728 PAGSPAPSPSPGGDD dbPAF 730 GSPAPSPSPGGDDKP dbPAF 74 KALCKRKSWMSKVFS dbPAF 774 SQWPGPASPPRVPPR dbPAF 785 VPPREPLSPQGSRTP dbPAF 789 EPLSPQGSRTPSPLV dbPAF 808 SPLPPRLSSSPGKTM dbPAF 810 LPPRLSSSPGKTMPT dbPAF 818 PGKTMPTTQSFASDP dbPAF 827 SFASDPKYATPQVIQ dbPAF 829 ASDPKYATPQVIQAP dbPAF 855 VRDGKKVSSTHYYLL dbPAF 856 RDGKKVSSTHYYLLP dbPAF 857 DGKKVSSTHYYLLPE dbPAF 859 KKVSSTHYYLLPERP dbPAF 860 KVSSTHYYLLPERPS dbPAF 867 YLLPERPSYLERYQR dbPAF 868 LLPERPSYLERYQRF dbPAF 872 RPSYLERYQRFLREA dbPAF 881 RFLREAQSPEEPTPL dbPAF 925 DPKANFSTNNSNPGA dbPAF 928 ANFSTNNSNPGARPP dbPAF
Ubiquitination (count: 9) (view all)	100 HSQSTFRKTSPAPGG PLMD 1011 RPRGECHKVLEMFDW PLMD 1037 SWGPAHHKR****** PLMD 131 KDLRLLEKLGDGSFG PLMD 48 VKNEDLEKIGMGRPG PLMD 539 FKRLGLRKPGLPRGL PLMD 813 RLSSSPGKTMPTTQS PLMD 826 QSFASDPKYATPQVI PLMD 853 PIVRDGKKVSSTHYY PLMD

PTM

Modification Sites

Phosphorylation
(count: 39)
(view all)

101       SQSTFRKTSPAPGGP     dbPAF
102       QSTFRKTSPAPGGPA     dbPAF
136       LEKLGDGSFGVVRRG     dbPAF
149       RGEWDAPSGKTVSVA     dbPAF
193       HRNLIRLYGVVLTPP     dbPAF
205       TPPMKMVTELAPLGS     dbPAF

Ubiquitination
(count: 9)
(view all)

100       HSQSTFRKTSPAPGG     PLMD
1011      RPRGECHKVLEMFDW     PLMD
1037      SWGPAHHKR******     PLMD
131       KDLRLLEKLGDGSFG     PLMD
48        VKNEDLEKIGMGRPG     PLMD
539       FKRLGLRKPGLPRGL     PLMD

※ Protein-Protein Interaction

Network

Interaction

Source

P00533	Q07912	IntAct;HPRD
P46108	Q07912	MINT
P56945	Q07912	MINT
Q07912	Q9NZC7	HPRD

PTMD Annotation Information

※ Protein Information

Functional Description

Fasta Sequence

※ PTM-Disease Association

※ Disease Cross-ref Annotation

※ PTM Sites

※ Protein-Protein Interaction