P35570

※ Protein Information

Tag	Content
UniProt Accession	IRS1_RAT; P35570;
Entrez ID	25467
GenBank Protein ID
GenBank Nucleotide ID
Protein Name	Insulin receptor substrate 1 (IRS-1) (pp185)
Gene Name	Irs1; Irs-1
Organism	Rattus norvegicus
NCBI Taxa ID	10116
Functional Description	May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. Functional Description May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Sequence (Fasta)	MASPPDTDGF SDVRKVGYLR KPKSMHKRFF VLRAASEAGG PARLEYYENE KKWRHKSSAP 60 KRSIPLESCF NINKRADSKN KHLVALYTRD EHFAIAADSE AEQDSWYQAL LQLHNRAKAH 120 HDGAGGGCGG SCSGSSGVGE AGEDLSYDTG PGPAFKEVWQ VILKPKGLGQ TKNLIGIYRL 180 CLTSKTISFV KLNSEAAAVV LQLMNIRRCG HSENFFFIEV GRSAVTGPGE FWMQVDDSVV 240 AQNMHETILE AMRAMSDEFR PRTKSQSSSS CSNPISVPLR RHHLNNPPPS QVGLTRRSRT 300 ESITATSPAS MVGGKPGSFR VRASSDGEGT MSRPASVDGS PVSPSTNRTH AHRHRGSSRL 360 HPPLNHSRSI PMPSSRCSPS ATSPVSLSSS STSGHGSTSD CLFPRRSSAS VSGSPSDGGF 420 ISSDEYGSSP CDFRSSFRSV TPDSLGHTPP ARGEEELSNY ICMGGKGAST LTAPNGHYIL 480 SRGGNGHRYI PGATMGTSPA LTGDEAAGAA DLDNRFRKRT HSAGTSPTIS HQKTPSQSSV 540 VSIEEYTEMM PAAYPPGGGS GGRLPGYRHS AFVPTHSYPE EGLEMHHLER RGGHHRPDSS 600 NLHTDDGYMP MSPGVAPVPS NRKGNGDYMP MSPKSVSAPQ QIINPIRRHP QRVDPNGYMM 660 MSPSGSCSPD IGGGSCSSSS ISAAPSGSSY GKPWTNGVGG HHTHALPHAK PPVESGGGKL 720 LPCTGDYMNM SPVGDSNTSS PSECYYGPED PQHKPVLSYY SLPRSFKHTQ RPGEPEEGAR 780 HQHLRLSSSS GRLRYTATAE DSSSSTSSDS LGGGYCGARP ESSVTHPHHH ALQPHLPRKV 840 DTAAQTNSRL ARPTRLSLGD PKASTLPRVR EQQQQQQQQQ QSSLHPPEPK SPGEYVNIEF 900 GSGQPGYLAG PATSRSSPSV RCLPQLHPAP REETGSEEYM NMDLGPGRRA TWQESGGVEL 960 GRVGPAPPGA ASICRPTRSV PNSRGDYMTM QIGCPRQSYV DTSPVAPVSY ADMRTGIAAE 1020 KVSLPRTTGA APPPSSTASA SASVTPQGAA EQAAHSSLLG GPQGPGGMSA FTRVNLSPNH 1080 NQSAKVIRAD TQGCRRRHSS ETFSAPTRAA NTVSFGAGAA GGGSGGGSED VKRHSSASFE 1140 NVWLRPGDLG GASKESAPGC GAAGGLEKSL NYIDLDLVKD VKQHPQDCPS QQQSLPPPPP 1200 HQPLGSNEGS SPRRSSEDLS TYASINFQKQ PEDRQ 1236 Fasta Sequence >P35570\|Irs1; Irs-1\|Rattus norvegicus (Rat) MASPPDTDGFSDVRKVGYLRKPKSMHKRFFVLRAASEAGGPARLEYYENEKKWRHKSSAPKRSIPLESCFNINKRADSKNKHLVALYTRDEHFAIAADSEAEQDSWYQALLQLHNRAKAHHDGAGGGCGGSCSGSSGVGEAGEDLSYDTGPGPAFKEVWQVILKPKGLGQTKNLIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIRRCGHSENFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSDEFRPRTKSQSSSSCSNPISVPLRRHHLNNPPPSQVGLTRRSRTESITATSPASMVGGKPGSFRVRASSDGEGTMSRPASVDGSPVSPSTNRTHAHRHRGSSRLHPPLNHSRSIPMPSSRCSPSATSPVSLSSSSTSGHGSTSDCLFPRRSSASVSGSPSDGGFISSDEYGSSPCDFRSSFRSVTPDSLGHTPPARGEEELSNYICMGGKGASTLTAPNGHYILSRGGNGHRYIPGATMGTSPALTGDEAAGAADLDNRFRKRTHSAGTSPTISHQKTPSQSSVVSIEEYTEMMPAAYPPGGGSGGRLPGYRHSAFVPTHSYPEEGLEMHHLERRGGHHRPDSSNLHTDDGYMPMSPGVAPVPSNRKGNGDYMPMSPKSVSAPQQIINPIRRHPQRVDPNGYMMMSPSGSCSPDIGGGSCSSSSISAAPSGSSYGKPWTNGVGGHHTHALPHAKPPVESGGGKLLPCTGDYMNMSPVGDSNTSSPSECYYGPEDPQHKPVLSYYSLPRSFKHTQRPGEPEEGARHQHLRLSSSSGRLRYTATAEDSSSSTSSDSLGGGYCGARPESSVTHPHHHALQPHLPRKVDTAAQTNSRLARPTRLSLGDPKASTLPRVREQQQQQQQQQQSSLHPPEPKSPGEYVNIEFGSGQPGYLAGPATSRSSPSVRCLPQLHPAPREETGSEEYMNMDLGPGRRATWQESGGVELGRVGPAPPGAASICRPTRSVPNSRGDYMTMQIGCPRQSYVDTSPVAPVSYADMRTGIAAEKVSLPRTTGAAPPPSSTASASASVTPQGAAEQAAHSSLLGGPQGPGGMSAFTRVNLSPNHNQSAKVIRADTQGCRRRHSSETFSAPTRAANTVSFGAGAAGGGSGGGSEDVKRHSSASFENVWLRPGDLGGASKESAPGCGAAGGLEKSLNYIDLDLVKDVKQHPQDCPSQQQSLPPPPPHQPLGSNEGSSPRRSSEDLSTYASINFQKQPEDRQ

※ PTM-Disease Association

Num	PTM	Disease	Type	PTM Site	PMID
1	Phosphorylation	MCA sarcoma	A		10080958
1	[Reference]: Inhibition of IRS-1 phosphorylation and the alterations of GLUT4 in isolated adipocytes from cachectic tumor-bearing rats.
2	Phosphorylation	Cancer cachexia/Cachexia/Cachectic tumor	A		10080958
2	[Reference]: These data suggest that the insulin resistance seen in adiposecells of these tumor-bearing rats was caused in part by a decreased amount of GLUT4 protein in both basal and insulin-stimulated states resulting from the selective inhibition of insulin-stimulated phosphorylation of IRS-1.
3	Phosphorylation	Hepatocellular carcinoma/Hepatocarcinoma/Hepatoma	U		2339989
3	[Reference]: Concanavalin A (ConA) stimulated the phosphorylation of the beta-subunit of the insulin receptor and an Mr-185,000 protein on serine and tyrosine residues in intact H-36 rat hepatoma cells.
4	Serine Phosphorylation	Diabetes mellitus	U	S1100	17709744
4	[Reference]: Identification of IRS-1 Ser-1101 as a target of S6K1 in nutrient- and obesity-induced insulin resistance.

※ Disease Cross-ref Annotation

Database	Annotation
CTD (Curated) (count: 13) (view all)	MESH:D006528 ; Carcinoma, Hepatocellular MESH:D002471 ; Cell Transformation, Neoplastic MESH:D003324 ; Coronary Artery Disease MESH:D003327 ; Coronary Disease MESH:D003920 ; Diabetes Mellitus MESH:D003921 ; Diabetes Mellitus, Experimental MESH:D003924 ; Diabetes Mellitus, Type 2 MESH:D006946 ; Hyperinsulinism MESH:D006949 ; Hyperlipidemias MESH:D007333 ; Insulin Resistance MESH:D009765 ; Obesity MESH:D011230 ; Precancerous Conditions MESH:D011471 ; Prostatic Neoplasms

Database

Annotation

CTD (Curated)
(count: 13)
(view all)

MESH:D006528 ; Carcinoma, Hepatocellular
MESH:D002471 ; Cell Transformation, Neoplastic
MESH:D003324 ; Coronary Artery Disease
MESH:D003327 ; Coronary Disease
MESH:D003920 ; Diabetes Mellitus
MESH:D003921 ; Diabetes Mellitus, Experimental

※ PTM Sites

PTM	Modification Sites
Phosphorylation (count: 60) (view all)	1003 RQSYVDTSPVAPVSY dbPAF 1009 TSPVAPVSYADMRTG dbPAF 1041 PSSTASASASVTPQG dbPAF 1045 ASASASVTPQGAAEQ dbPAF 1077 AFTRVNLSPNHNQSA dbPAF 1099 QGCRRRHSSETFSAP dbPAF 1100 GCRRRHSSETFSAPT dbPAF 1135 SEDVKRHSSASFENV dbPAF 1169 AAGGLEKSLNYIDLD dbPAF 1172 GLEKSLNYIDLDLVK dbPAF 1215 EGSSPRRSSEDLSTY dbPAF 1216 GSSPRRSSEDLSTYA dbPAF 1222 SSEDLSTYASINFQK dbPAF 24 GYLRKPKSMHKRFFV dbPAF 265 EFRPRTKSQSSSSCS dbPAF 270 TKSQSSSSCSNPISV dbPAF 3 *****MASPPDTDGF dbPAF 302 TRRSRTESITATSPA dbPAF 307 TESITATSPASMVGG dbPAF 318 MVGGKPGSFRVRASS dbPAF 324 GSFRVRASSDGEGTM dbPAF 325 SFRVRASSDGEGTMS dbPAF 332 SDGEGTMSRPASVDG dbPAF 336 GTMSRPASVDGSPVS dbPAF 343 SVDGSPVSPSTNRTH dbPAF 357 HAHRHRGSSRLHPPL dbPAF 358 AHRHRGSSRLHPPLN dbPAF 369 PPLNHSRSIPMPSSR dbPAF 407 DCLFPRRSSASVSGS dbPAF 408 CLFPRRSSASVSGSP dbPAF 412 RRSSASVSGSPSDGG dbPAF 441 RSSFRSVTPDSLGHT dbPAF 444 FRSVTPDSLGHTPPA dbPAF 448 TPDSLGHTPPARGEE dbPAF 460 GEEELSNYICMGGKG dbPAF 498 PGATMGTSPALTGDE dbPAF 502 MGTSPALTGDEAAGA dbPAF 522 RFRKRTHSAGTSPTI dbPAF 526 RTHSAGTSPTISHQK dbPAF 528 HSAGTSPTISHQKTP dbPAF 570 RLPGYRHSAFVPTHS dbPAF 599 GGHHRPDSSNLHTDD dbPAF 600 GHHRPDSSNLHTDDG dbPAF 604 PDSSNLHTDDGYMPM dbPAF 608 NLHTDDGYMPMSPGV dbPAF 612 DDGYMPMSPGVAPVP dbPAF 628 NRKGNGDYMPMSPKS dbPAF 632 NGDYMPMSPKSVSAP dbPAF 635 YMPMSPKSVSAPQQI dbPAF 658 QRVDPNGYMMMSPSG dbPAF 662 PNGYMMMSPSGSCSP dbPAF 727 LLPCTGDYMNMSPVG dbPAF 731 TGDYMNMSPVGDSNT dbPAF 761 KPVLSYYSLPRSFKH dbPAF 789 QHLRLSSSSGRLRYT dbPAF 891 LHPPEPKSPGEYVNI dbPAF 895 EPKSPGEYVNIEFGS dbPAF 939 EETGSEEYMNMDLGP dbPAF 987 VPNSRGDYMTMQIGC dbPAF 99 HFAIAADSEAEQDSW dbPAF
Acetylation (count: 1)	315 PASMVGGKPGSFRVR PLMD

PTM

Modification Sites

Phosphorylation
(count: 60)
(view all)

1003      RQSYVDTSPVAPVSY     dbPAF
1009      TSPVAPVSYADMRTG     dbPAF
1041      PSSTASASASVTPQG     dbPAF
1045      ASASASVTPQGAAEQ     dbPAF
1077      AFTRVNLSPNHNQSA     dbPAF
1099      QGCRRRHSSETFSAP     dbPAF

Acetylation
(count: 1)

315 PASMVGGKPGSFRVR PLMD

PTMD Annotation Information

※ Protein Information

Functional Description

Fasta Sequence

※ PTM-Disease Association

※ Disease Cross-ref Annotation

※ PTM Sites