P35569

※ Protein Information

Tag	Content
UniProt Accession	IRS1_MOUSE; P35569;
Entrez ID	16367
GenBank Protein ID
GenBank Nucleotide ID
Protein Name	Insulin receptor substrate 1 (IRS-1)
Gene Name	Irs1; Irs-1
Organism	Mus musculus
NCBI Taxa ID	10090
Functional Description	May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity). Functional Description May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
Sequence (Fasta)	MASPPDTDGF SDVRKVGYLR KPKSMHKRFF VLRAASEAGG PARLEYYENE KKWRHKSSAP 60 KRSIPLESCF NINKRADSKN KHLVALYTRD EHFAIAADSE AEQDSWYQAL LQLHNRAKAH 120 HDGAGGGCGG SCSGSSGVGE AGEDLSYDTG PGPAFKEVWQ VILKPKGLGQ TKNLIGIYRL 180 CLTSKTISFV KLNSEAAAVV LQLMNIRRCG HSENFFFIEV GRSAVTGPGE FWMQVDDSVV 240 AQNMHETILE AMRAMSDEFR PRSKSQSSSS CSNPISVPLR RHHLNNPPPS QVGLTRRSRT 300 ESITATSPAS MVGGKPGSFR VRASSDGEGT MSRPASVDGS PVSPSTNRTH AHRHRGSSRL 360 HPPLNHSRSI PMPSSRCSPS ATSPVSLSSS STSGHGSTSD CLFPRRSSAS VSGSPSDGGF 420 ISSDEYGSSP CDFRSSFRSV TPDSLGHTPP ARGEEELSNY ICMGGKGAST LAAPNGHYIL 480 SRGGNGHRYI PGANLGTSPA LPGDEAAGAA DLDNRFRKRT HSAGTSPTIS HQKTPSQSSV 540 ASIEEYTEMM PAAYPPGGGS GGRLPGYRHS AFVPTHSYPE EGLEMHHLER RGGHHRPDTS 600 NLHTDDGYMP MSPGVAPVPS NRKGNGDYMP MSPKSVSAPQ QIINPIRRHP QRVDPNGYMM 660 MSPSGSCSPD IGGGSSSSSS ISAAPSGSSY GKPWTNGVGG HHTHALPHAK PPVESGGGKL 720 LPCTGDYMNM SPVGDSNTSS PSECYYGPED PQHKPVLSYY SLPRSFKHTQ RPGEPEEGAR 780 HQHLRLSSSS GRLRYTATAE DSSSSTSSDS LGGGYCGARP ESSLTHPHHH VLQPHLPRKV 840 DTAAQTNSRL ARPTRLSLGD PKASTLPRVR EQQQQQQSSL HPPEPKSPGE YVNIEFGSGQ 900 PGYLAGPATS RSSPSVRCPP QLHPAPREET GSEEYMNMDL GPGRRATWQE SGGVELGRIG 960 PAPPGSATVC RPTRSVPNSR GDYMTMQIGC PRQSYVDTSP VAPVSYADMR TGIAAEKASL 1020 PRPTGAAPPP SSTASSSASV TPQGATAEQA THSSLLGGPQ GPGGMSAFTR VNLSPNHNQS 1080 AKVIRADTQG CRRRHSSETF SAPTRAGNTV PFGAGAAVGG SGGGGGGGSE DVKRHSSASF 1140 ENVWLRPGDL GGVSKESAPV CGAAGGLEKS LNYIDLDLAK EHSQDCPSQQ QSLPPPPPHQ 1200 PLGSNEGNSP RRSSEDLSNY ASISFQKQPE DRQ 1234 Fasta Sequence >P35569\|Irs1; Irs-1\|Mus musculus (Mouse) MASPPDTDGFSDVRKVGYLRKPKSMHKRFFVLRAASEAGGPARLEYYENEKKWRHKSSAPKRSIPLESCFNINKRADSKNKHLVALYTRDEHFAIAADSEAEQDSWYQALLQLHNRAKAHHDGAGGGCGGSCSGSSGVGEAGEDLSYDTGPGPAFKEVWQVILKPKGLGQTKNLIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIRRCGHSENFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSDEFRPRSKSQSSSSCSNPISVPLRRHHLNNPPPSQVGLTRRSRTESITATSPASMVGGKPGSFRVRASSDGEGTMSRPASVDGSPVSPSTNRTHAHRHRGSSRLHPPLNHSRSIPMPSSRCSPSATSPVSLSSSSTSGHGSTSDCLFPRRSSASVSGSPSDGGFISSDEYGSSPCDFRSSFRSVTPDSLGHTPPARGEEELSNYICMGGKGASTLAAPNGHYILSRGGNGHRYIPGANLGTSPALPGDEAAGAADLDNRFRKRTHSAGTSPTISHQKTPSQSSVASIEEYTEMMPAAYPPGGGSGGRLPGYRHSAFVPTHSYPEEGLEMHHLERRGGHHRPDTSNLHTDDGYMPMSPGVAPVPSNRKGNGDYMPMSPKSVSAPQQIINPIRRHPQRVDPNGYMMMSPSGSCSPDIGGGSSSSSSISAAPSGSSYGKPWTNGVGGHHTHALPHAKPPVESGGGKLLPCTGDYMNMSPVGDSNTSSPSECYYGPEDPQHKPVLSYYSLPRSFKHTQRPGEPEEGARHQHLRLSSSSGRLRYTATAEDSSSSTSSDSLGGGYCGARPESSLTHPHHHVLQPHLPRKVDTAAQTNSRLARPTRLSLGDPKASTLPRVREQQQQQQSSLHPPEPKSPGEYVNIEFGSGQPGYLAGPATSRSSPSVRCPPQLHPAPREETGSEEYMNMDLGPGRRATWQESGGVELGRIGPAPPGSATVCRPTRSVPNSRGDYMTMQIGCPRQSYVDTSPVAPVSYADMRTGIAAEKASLPRPTGAAPPPSSTASSSASVTPQGATAEQATHSSLLGGPQGPGGMSAFTRVNLSPNHNQSAKVIRADTQGCRRRHSSETFSAPTRAGNTVPFGAGAAVGGSGGGGGGGSEDVKRHSSASFENVWLRPGDLGGVSKESAPVCGAAGGLEKSLNYIDLDLAKEHSQDCPSQQQSLPPPPPHQPLGSNEGNSPRRSSEDLSNYASISFQKQPEDRQ

※ PTM-Disease Association

Num	PTM	Disease	Type	PTM Site	PMID
1	Serine Phosphorylation	Obesity	U	S265	18952604
1	[Reference]: This led to phosphorylation of IRS-1 by S6K1 at multiple serine residues including Ser-270, Ser-307, Ser-636, and Ser-1101 in human IRS-1 (Ser-265, Ser-302, Ser-632, and Ser-1097, in rodent IRS-1). Direct phosphorylation of these sites by S6K1 was observed in an in vitro kinase assay using purified IRS-1 and S6K1. Phosphorylation of all these serines was increased in the adipose tissue of obese mice.
2	Serine Phosphorylation	Obesity	U	S302	18952604
2	[Reference]: This led to phosphorylation of IRS-1 by S6K1 at multiple serine residues including Ser-270, Ser-307, Ser-636, and Ser-1101 in human IRS-1. Phosphorylation of all these serines was increased in the adipose tissue of obese mice.
3	Serine Phosphorylation	Diabetes mellitus	U	S307	16777975
3	[Reference]: Insulin resistance accelerates muscle protein degradation: Activation of the ubiquitin-proteasome pathway by defects in muscle cell signaling.
4	Serine Phosphorylation	Obesity	U	S632	18952604
4	[Reference]: S6K1 was phosphorylated at Thr-389 in response to TNF-alpha. This led to phosphorylation of IRS-1 by S6K1 at multiple serine residues including Ser-270, Ser-307, Ser-636, and Ser-1101 in human IRS-1 (Ser-265, Ser-302, Ser-632, and Ser-1097, in rodent IRS-1). Direct phosphorylation of these sites by S6K1 was observed in an in vitro kinase assay using purified IRS-1 and S6K1. Phosphorylation of all these serines was increased in the adipose tissue of obese mice.?
5	Serine Phosphorylation	Obesity	U	S1097	18952604
5	[Reference]: S6K1 was phosphorylated at Thr-389 in response to TNF-alpha. This led to phosphorylation of IRS-1 by S6K1 at multiple serine residues including Ser-270, Ser-307, Ser-636, and Ser-1101 in human IRS-1 (Ser-265, Ser-302, Ser-632, and Ser-1097, in rodent IRS-1). Direct phosphorylation of these sites by S6K1 was observed in an in vitro kinase assay using purified IRS-1 and S6K1. Phosphorylation of all these serines was increased in the adipose tissue of obese mice.?

※ Disease Cross-ref Annotation

Database	Annotation
CTD (Curated) (count: 13) (view all)	MESH:D006528 ; Carcinoma, Hepatocellular MESH:D002471 ; Cell Transformation, Neoplastic MESH:D003324 ; Coronary Artery Disease MESH:D003327 ; Coronary Disease MESH:D003920 ; Diabetes Mellitus MESH:D003921 ; Diabetes Mellitus, Experimental MESH:D003924 ; Diabetes Mellitus, Type 2 MESH:D006946 ; Hyperinsulinism MESH:D006949 ; Hyperlipidemias MESH:D007333 ; Insulin Resistance MESH:D009765 ; Obesity MESH:D011230 ; Precancerous Conditions MESH:D011471 ; Prostatic Neoplasms

Database

Annotation

CTD (Curated)
(count: 13)
(view all)

MESH:D006528 ; Carcinoma, Hepatocellular
MESH:D002471 ; Cell Transformation, Neoplastic
MESH:D003324 ; Coronary Artery Disease
MESH:D003327 ; Coronary Disease
MESH:D003920 ; Diabetes Mellitus
MESH:D003921 ; Diabetes Mellitus, Experimental

※ PTM Sites

PTM	Modification Sites
Phosphorylation (count: 96) (view all)	1074 AFTRVNLSPNHNQSA dbPAF 1096 QGCRRRHSSETFSAP dbPAF 1097 GCRRRHSSETFSAPT dbPAF 1099 RRRHSSETFSAPTRA dbPAF 1101 RHSSETFSAPTRAGN dbPAF 1104 SETFSAPTRAGNTVP dbPAF 1136 SEDVKRHSSASFENV dbPAF 1137 EDVKRHSSASFENVW dbPAF 1139 VKRHSSASFENVWLR dbPAF 1170 AAGGLEKSLNYIDLD dbPAF 1173 GLEKSLNYIDLDLAK dbPAF 1209 LGSNEGNSPRRSSED dbPAF 1213 EGNSPRRSSEDLSNY dbPAF 1214 GNSPRRSSEDLSNYA dbPAF 1218 RRSSEDLSNYASISF dbPAF 1220 SSEDLSNYASISFQK dbPAF 131 AGGGCGGSCSGSSGV dbPAF 147 EAGEDLSYDTGPGPA dbPAF 263 SDEFRPRSKSQSSSS dbPAF 265 EFRPRSKSQSSSSCS dbPAF 267 RPRSKSQSSSSCSNP dbPAF 268 PRSKSQSSSSCSNPI dbPAF 270 SKSQSSSSCSNPISV dbPAF 272 SQSSSSCSNPISVPL dbPAF 298 QVGLTRRSRTESITA dbPAF 3 *****MASPPDTDGF dbPAF 300 GLTRRSRTESITATS dbPAF 302 TRRSRTESITATSPA dbPAF 304 RSRTESITATSPASM dbPAF 306 RTESITATSPASMVG dbPAF 307 TESITATSPASMVGG dbPAF 310 ITATSPASMVGGKPG dbPAF 318 MVGGKPGSFRVRASS dbPAF 324 GSFRVRASSDGEGTM dbPAF 325 SFRVRASSDGEGTMS dbPAF 330 ASSDGEGTMSRPASV dbPAF 332 SDGEGTMSRPASVDG dbPAF 336 GTMSRPASVDGSPVS dbPAF 340 RPASVDGSPVSPSTN dbPAF 343 SVDGSPVSPSTNRTH dbPAF 345 DGSPVSPSTNRTHAH dbPAF 346 GSPVSPSTNRTHAHR dbPAF 369 PPLNHSRSIPMPSSR dbPAF 378 PMPSSRCSPSATSPV dbPAF 383 RCSPSATSPVSLSSS dbPAF 407 DCLFPRRSSASVSGS dbPAF 408 CLFPRRSSASVSGSP dbPAF 410 FPRRSSASVSGSPSD dbPAF 412 RRSSASVSGSPSDGG dbPAF 414 SSASVSGSPSDGGFI dbPAF 416 ASVSGSPSDGGFISS dbPAF 422 PSDGGFISSDEYGSS dbPAF 423 SDGGFISSDEYGSSP dbPAF 426 GFISSDEYGSSPCDF dbPAF 428 ISSDEYGSSPCDFRS dbPAF 429 SSDEYGSSPCDFRSS dbPAF 439 DFRSSFRSVTPDSLG dbPAF 441 RSSFRSVTPDSLGHT dbPAF 444 FRSVTPDSLGHTPPA dbPAF 448 TPDSLGHTPPARGEE dbPAF 46 GGPARLEYYENEKKW dbPAF 460 GEEELSNYICMGGKG dbPAF 47 GPARLEYYENEKKWR dbPAF 478 LAAPNGHYILSRGGN dbPAF 498 PGANLGTSPALPGDE dbPAF 520 DNRFRKRTHSAGTSP dbPAF 522 RFRKRTHSAGTSPTI dbPAF 525 KRTHSAGTSPTISHQ dbPAF 526 RTHSAGTSPTISHQK dbPAF 530 AGTSPTISHQKTPSQ dbPAF 534 PTISHQKTPSQSSVA dbPAF 536 ISHQKTPSQSSVASI dbPAF 542 PSQSSVASIEEYTEM dbPAF 577 SAFVPTHSYPEEGLE dbPAF 599 GGHHRPDTSNLHTDD dbPAF 600 GHHRPDTSNLHTDDG dbPAF 604 PDTSNLHTDDGYMPM dbPAF 608 NLHTDDGYMPMSPGV dbPAF 612 DDGYMPMSPGVAPVP dbPAF 628 NRKGNGDYMPMSPKS dbPAF 632 NGDYMPMSPKSVSAP dbPAF 635 YMPMSPKSVSAPQQI dbPAF 637 PMSPKSVSAPQQIIN dbPAF 662 PNGYMMMSPSGSCSP dbPAF 731 TGDYMNMSPVGDSNT dbPAF 761 KPVLSYYSLPRSFKH dbPAF 789 QHLRLSSSSGRLRYT dbPAF 878 EQQQQQQSSLHPPEP dbPAF 887 LHPPEPKSPGEYVNI dbPAF 891 EPKSPGEYVNIEFGS dbPAF 932 APREETGSEEYMNMD dbPAF 935 EETGSEEYMNMDLGP dbPAF 983 VPNSRGDYMTMQIGC dbPAF 99 HFAIAADSEAEQDSW dbPAF 998 PRQSYVDTSPVAPVS dbPAF 999 RQSYVDTSPVAPVSY dbPAF
Acetylation (count: 1)	315 PASMVGGKPGSFRVR PLMD

PTM

Modification Sites

Phosphorylation
(count: 96)
(view all)

1074      AFTRVNLSPNHNQSA     dbPAF
1096      QGCRRRHSSETFSAP     dbPAF
1097      GCRRRHSSETFSAPT     dbPAF
1099      RRRHSSETFSAPTRA     dbPAF
1101      RHSSETFSAPTRAGN     dbPAF
1104      SETFSAPTRAGNTVP     dbPAF

Acetylation
(count: 1)

315 PASMVGGKPGSFRVR PLMD

※ Protein-Protein Interaction

Network

Interaction

Source

P35235	P35569	MINT
P35569	Q01097	IntAct
P35569	Q923E4	IntAct

PTMD Annotation Information

※ Protein Information

Functional Description

Fasta Sequence

※ PTM-Disease Association

※ Disease Cross-ref Annotation

※ PTM Sites

※ Protein-Protein Interaction