P29474

※ Protein Information

Tag	Content
UniProt Accession	NOS3_HUMAN; P29474;
Entrez ID	4846
GenBank Protein ID	NM_000603.4; NM_001160109.1; NM_001160110.1; NM_001160111.1; XM_017012232.1;
GenBank Nucleotide ID	NP_000594.2; NP_001153581.1; NP_001153582.1; NP_001153583.1; XP_016867721.1;
Protein Name	Nitric oxide synthase, endothelial (EC 1.14.13.39) (Constitutive NOS) (cNOS) (EC-NOS) (Endothelial NOS) (eNOS) (NOS type III) (NOSIII)
Gene Name	NOS3
Organism	Homo sapiens
NCBI Taxa ID	9606
Functional Description	Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.;Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. Functional Description Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.;Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Sequence (Fasta)	MGNLKSVAQE PGPPCGLGLG LGLGLCGKQG PATPAPEPSR APASLLPPAP EHSPPSSPLT 60 QPPEGPKFPR VKNWEVGSIT YDTLSAQAQQ DGPCTPRRCL GSLVFPRKLQ GRPSPGPPAP 120 EQLLSQARDF INQYYSSIKR SGSQAHEQRL QEVEAEVAAT GTYQLRESEL VFGAKQAWRN 180 APRCVGRIQW GKLQVFDARD CRSAQEMFTY ICNHIKYATN RGNLRSAITV FPQRCPGRGD 240 FRIWNSQLVR YAGYRQQDGS VRGDPANVEI TELCIQHGWT PGNGRFDVLP LLLQAPDEPP 300 ELFLLPPELV LEVPLEHPTL EWFAALGLRW YALPAVSNML LEIGGLEFPA APFSGWYMST 360 EIGTRNLCDP HRYNILEDVA VCMDLDTRTT SSLWKDKAAV EINVAVLHSY QLAKVTIVDH 420 HAATASFMKH LENEQKARGG CPADWAWIVP PISGSLTPVF HQEMVNYFLS PAFRYQPDPW 480 KGSAAKGTGI TRKKTFKEVA NAVKISASLM GTVMAKRVKA TILYGSETGR AQSYAQQLGR 540 LFRKAFDPRV LCMDEYDVVS LEHETLVLVV TSTFGNGDPP ENGESFAAAL MEMSGPYNSS 600 PRPEQHKSYK IRFNSISCSD PLVSSWRRKR KESSNTDSAG ALGTLRFCVF GLGSRAYPHF 660 CAFARAVDTR LEELGGERLL QLGQGDELCG QEEAFRGWAQ AAFQAACETF CVGEDAKAAA 720 RDIFSPKRSW KRQRYRLSAQ AEGLQLLPGL IHVHRRKMFQ ATIRSVENLQ SSKSTRATIL 780 VRLDTGGQEG LQYQPGDHIG VCPPNRPGLV EALLSRVEDP PAPTEPVAVE QLEKGSPGGP 840 PPGWVRDPRL PPCTLRQALT FFLDITSPPS PQLLRLLSTL AEEPREQQEL EALSQDPRRY 900 EEWKWFRCPT LLEVLEQFPS VALPAPLLLT QLPLLQPRYY SVSSAPSTHP GEIHLTVAVL 960 AYRTQDGLGP LHYGVCSTWL SQLKPGDPVP CFIRGAPSFR LPPDPSLPCI LVGPGTGIAP 1020 FRGFWQERLH DIESKGLQPT PMTLVFGCRC SQLDHLYRDE VQNAQQRGVF GRVLTAFSRE 1080 PDNPKTYVQD ILRTELAAEV HRVLCLERGH MFVCGDVTMA TNVLQTVQRI LATEGDMELD 1140 EAGDVIGVLR DQQRYHEDIF GLTLRTQEVT SRIRTQSFSL QERQLRGAVP WAFDPPGSDT 1200 NSP 1204 Fasta Sequence >P29474\|NOS3\|Homo sapiens (Human) MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDEPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMFQATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP

※ PTM-Disease Association

Num	PTM	Disease	Cell Type	Type	PTM Site	PMID
1	Serine Phosphorylation	Diabetes mellitus	tumor tissue	U	S1177	23204109
1	[Reference]: Unexpectedly, we observed 1.7-fold higher basal endothelial nitric oxide synthase (eNOS) phosphorylation at serine 1177 in patients with diabetes mellitus (P=0.007)

※ Disease Cross-ref Annotation

Database	Annotation
CTD (Curated) (count: 58) (view all)	MESH:D000037 ; Abruptio Placentae MESH:D058186 ; Acute Kidney Injury MESH:D000230 ; Adenocarcinoma MESH:D000544 ; Alzheimer Disease MESH:D001161 ; Arteriosclerosis MESH:D050197 ; Atherosclerosis MESH:D001281 ; Atrial Fibrillation MESH:D001943 ; Breast Neoplasms MESH:D006332 ; Cardiomegaly MESH:D002318 ; Cardiovascular Diseases MESH:D002544 ; Cerebral Infarction MESH:D002971 ; Cleft Lip MESH:D002972 ; Cleft Palate MESH:D003324 ; Coronary Artery Disease MESH:D003329 ; Coronary Vasospasm MESH:D003921 ; Diabetes Mellitus, Experimental MESH:D003922 ; Diabetes Mellitus, Type 1 MESH:D003924 ; Diabetes Mellitus, Type 2 MESH:D003925 ; Diabetic Angiopathies MESH:D003928 ; Diabetic Nephropathies MESH:D020345 ; Enterocolitis, Necrotizing MESH:D007172 ; Erectile Dysfunction MESH:D004938 ; Esophageal Neoplasms MESH:D005317 ; Fetal Growth Retardation MESH:D006333 ; Heart Failure MESH:D006548 ; Hernia, Diaphragmatic MESH:D006943 ; Hyperglycemia MESH:D006946 ; Hyperinsulinism MESH:D006949 ; Hyperlipidemias MESH:D006973 ; Hypertension MESH:D006975 ; Hypertension, Portal MESH:D046110 ; Hypertension, Pregnancy-Induced MESH:D006976 ; Hypertension, Pulmonary MESH:D006978 ; Hypertension, Renovascular MESH:D000860 ; Hypoxia MESH:D007248 ; Infertility, Male MESH:D007333 ; Insulin Resistance MESH:D007416 ; Intestinal Perforation MESH:D007511 ; Ischemia MESH:D008106 ; Liver Cirrhosis, Experimental MESH:D024821 ; Metabolic Syndrome MESH:D009203 ; Myocardial Infarction MESH:D017202 ; Myocardial Ischemia MESH:D015428 ; Myocardial Reperfusion Injury MESH:D009369 ; Neoplasms MESH:D011225 ; Pre-Eclampsia MESH:D011471 ; Prostatic Neoplasms MESH:D029424 ; Pulmonary Disease, Chronic Obstructive MESH:D011654 ; Pulmonary Edema MESH:D015427 ; Reperfusion Injury MESH:D018805 ; Sepsis MESH:D013274 ; Stomach Neoplasms MESH:D013276 ; Stomach Ulcer MESH:D017180 ; Tachycardia, Ventricular MESH:D014511 ; Uremia MESH:D014517 ; Ureteral Obstruction MESH:D018879 ; Ventricular Premature Complexes MESH:D015837 ; Vestibular Diseases
HGMD (count: 6)	CE004922; Coronary artery disease, association with; Repeat variations CG962921; Coronary artery disease, association with; Gross deletions CM981388; Coronary spasm, association with; Missense/nonsense CR067136; Limb deficiency defect, assoc. with ?; Regulatory CR994303; Coronary spasm, association with; Regulatory CS030992; Diabetes, type 2, association with; Splicing
GWASdb (count: 9) (view all)	rs1800783; Height; Null rs2070744; Hypertension (essential hypertension); hypertension rs3918226; Coronary heart disease; coronary artery disease rs3918226; Blood pressure; hypertension rs3918226; Hypertension (essential hypertension); hypertension rs1549758; Hypertension (essential hypertension); hypertension rs2853792; Hypertension (essential hypertension); hypertension rs3918186; Multiple complex diseases; Null rs3918198; Triglycerides; coronary artery disease\|lipid metabolism disorder\|arteriosclerosis

※ PTM Sites

PTM	Modification Sites
Phosphorylation (count: 23) (view all)	102 TPRRCLGSLVFPRKL dbPAF 1094 YVQDILRTELAAEVH dbPAF 114 RKLQGRPSPGPPAPE dbPAF 1175 EVTSRIRTQSFSLQE dbPAF 1177 TSRIRTQSFSLQERQ dbPAF 1179 RIRTQSFSLQERQLR dbPAF 1202 PPGSDTNSP****** dbPAF 210 SAQEMFTYICNHIKY dbPAF 33 CGKQGPATPAPEPSR dbPAF 495 TGITRKKTFKEVANA dbPAF 508 NAVKISASLMGTVMA dbPAF 512 ISASLMGTVMAKRVK dbPAF 53 LPPAPEHSPPSSPLT dbPAF 615 SYKIRFNSISCSDPL dbPAF 625 CSDPLVSSWRRKRKE dbPAF 633 WRRKRKESSNTDSAG dbPAF 638 KESSNTDSAGALGTL dbPAF 657 FGLGSRAYPHFCAFA dbPAF 738 KRQRYRLSAQAEGLQ dbPAF 81 WEVGSITYDTLSAQA dbPAF 83 VGSITYDTLSAQAQQ dbPAF 836 VEQLEKGSPGGPPPG dbPAF 854 DPRLPPCTLRQALTF dbPAF
Acetylation (count: 2)	494 GTGITRKKTFKEVAN PLMD 504 KEVANAVKISASLMG PLMD
Ubiquitination (count: 5)	1035 RLHDIESKGLQPTPM PLMD 1085 SREPDNPKTYVQDIL PLMD 773 VENLQSSKSTRATIL PLMD 834 VAVEQLEKGSPGGPP PLMD 904 PRRYEEWKWFRCPTL PLMD
Methylation (count: 1)	5 ***MGNLKSVAQEPG PLMD

※ Protein-Protein Interaction

Network

Interaction

Source

C9JP52	P29474	BioGRID
H0YGH4	P29474	IntAct
O43707	P29474	IntAct
O95967	P29474	IntAct
P03372	P29474	DIP
P07900	P29474	HPRD
P08047	P29474	HPRD
P11172	P29474	IntAct
P15428	P29474	BioGRID
P23560	P29474	HPRD
P24530	P29474	HPRD
P29474	P29474	HPRD
P29474	P30411	HPRD
P29474	P30419	HPRD
P29474	P30556	HPRD
P29474	P31749	HPRD
P29474	P35609	IntAct
P29474	P50502	IntAct
P29474	P62158	IntAct
P29474	P84243	IntAct
P29474	Q03135	HPRD
P29474	Q12805	IntAct
P29474	Q14576	IntAct
P29474	Q15172	HPRD
P29474	Q15717	IntAct
P29474	Q16543	IntAct
P29474	Q16891	IntAct
P29474	Q63ZY3	IntAct
P29474	Q6PKC3	IntAct
P29474	Q6WCQ1	IntAct
P29474	Q8IVI9	HPRD; IntAct; MINT
P29474	Q8TAG9	IntAct
P29474	Q92743	IntAct
P29474	Q92947	IntAct
P29474	Q96EB6	DIP
P29474	Q96EP0	IntAct
P29474	Q9BTU6	IntAct
P29474	Q9H0A6	IntAct
P29474	Q9Y2H9	IntAct
P29474	Q9Y314	HPRD
P29474	Q9Y3D6	IntAct

PTMD Annotation Information

※ Protein Information

Functional Description

Fasta Sequence

※ PTM-Disease Association

※ Disease Cross-ref Annotation

※ PTM Sites

※ Protein-Protein Interaction