P28352

PTMD Annotation Information


※ Protein Information

Tag Content
UniProt Accession APEX1_MOUSE; P28352;
Entrez ID 11792
GenBank Protein ID
GenBank Nucleotide ID
Protein Name DNA-(apurinic or apyrimidinic site) lyase (EC 3.1.-.-) (EC 4.2.99.18) (APEX nuclease) (APEN) (Apurinic-apyrimidinic endonuclease 1) (AP endonuclease 1) (REF-1) (Redox factor-1) [Cleaved into: DNA-(apurinic or apyrimidinic site) lyase, mitochondrial]
Gene Name Apex1; Ape; Apex; Ref1
Organism Mus musculus
NCBI Taxa ID 10090
Functional DescriptionMultifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribos(view all)
Sequence
(Fasta)
MPKRGKKAAA DDGEEPKSEP ETKKSKGAAK KTEKEAAGEG PVLYEDPPDQ KTSPSGKSAT 60
LKICSWNVDG LRAWIKKKGL DWVKEEAPDI LCLQETKCSE NKLPAELQEL PGLTHQYWSA 120
PSDKEGYSGV GLLSRQCPLK VSYGIGEEEH DQEGRVIVAE FESFVLVTAY VPNAGRGLVR 180
LEYRQRWDEA FRKFLKDLAS RKPLVLCGDL NVAHEEIDLR NPKGNKKNAG FTPQERQGFG 240
ELLQAVPLAD SFRHLYPNTA YAYTFWTYMM NARSKNVGWR LDYFLLSHSL LPALCDSKIR 300
SKALGSDHCP ITLYLAL 318

※ PTM-Disease Association

NumPTMDiseaseCell TypeTypePTM SitePMID
1Threonine PhosphorylationParkinson's diseaseUT23220473298
[Reference]: Here we demonstrate that the Cdk5 activator p35 interacts directly with apurinic/apyrimidinic endonuclease 1 (Ape1), a protein crucial for base excision repair (BER) following DNA damage. Cdk5 complexes phosphorylate Ape1 at Thr 232 and thereby reduces its apurinic/apyrimidinic (AP) endonuclease activity.?
2Threonine PhosphorylationAlzheimer's diseaseUT23220473298
[Reference]: Importantly, increased phosphorylated Ape1 was also observed in post-mortem brain tissue from patients with Parkinson's and Alzheimer's diseases, suggesting a potential link between Ape1 phosphorylation and the pathogenesis of neurodegenerative diseases.

※ Disease Cross-ref Annotation

DatabaseAnnotation
CTD (Curated)
(count: 10)
(view all)
MESH:D001930 ; Brain Injuries
MESH:D006528 ; Carcinoma, Hepatocellular
MESH:D018450 ; Disease Progression
MESH:D020022 ; Genetic Predisposition to Disease
MESH:D006331 ; Heart Diseases
MESH:D008545 ; Melanoma

※ PTM Sites

PTM Modification Sites
Phosphorylation
(count: 5)
127       APSDKEGYSGVGLLS     dbPAF
18        DDGEEPKSEPETKKS     dbPAF
22        EPKSEPETKKSKGAA     dbPAF
232       NKKNAGFTPQERQGF     dbPAF
53        DPPDQKTSPSGKSAT     dbPAF
Acetylation
(count: 4)
124       YWSAPSDKEGYSGVG     PLMD
196       EAFRKFLKDLASRKP     PLMD
202       LKDLASRKPLVLCGD     PLMD
34        GAAKKTEKEAAGEGP     PLMD
Ubiquitination
(count: 1)
227       RNPKGNKKNAGFTPQ     PLMD
Malonylation
(count: 1)
275       YMMNARSKNVGWRLD     PLMD