P27361

PTMD Annotation Information


※ Protein Information

Tag Content
UniProt Accession MK03_HUMAN; P27361;
Entrez ID 5595
GenBank Protein ID NM_001040056.2; NM_001109891.1; NM_002746.2;
GenBank Nucleotide ID NP_001035145.1; NP_001103361.1; NP_002737.2;
Protein Name Mitogen-activated protein kinase 3 (MAP kinase 3) (MAPK 3) (EC 2.7.11.24) (ERT2) (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin-stimulated MAP2 kinase) (MAP kinase isoform p44) (p44-MAPK) (Microtubule-associated protein 2 kinase) (p44-ERK1)
Gene Name MAPK3; ERK1; PRKM3
Organism Homo sapiens
NCBI Taxa ID 9606
Functional DescriptionSerine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade p(view all)
Sequence
(Fasta)
MAAAAAQGGG GGEPRRTEGV GPGVPGEVEM VKGQPFDVGP RYTQLQYIGE GAYGMVSSAY 60
DHVRKTRVAI KKISPFEHQT YCQRTLREIQ ILLRFRHENV IGIRDILRAS TLEAMRDVYI 120
VQDLMETDLY KLLKSQQLSN DHICYFLYQI LRGLKYIHSA NVLHRDLKPS NLLINTTCDL 180
KICDFGLARI ADPEHDHTGF LTEYVATRWY RAPEIMLNSK GYTKSIDIWS VGCILAEMLS 240
NRPIFPGKHY LDQLNHILGI LGSPSQEDLN CIINMKARNY LQSLPSKTKV AWAKLFPKSD 300
SKALDLLDRM LTFNPNKRIT VEEALAHPYL EQYYDPTDEP VAEEPFTFAM ELDDLPKERL 360
KELIFQETAR FQPGVLEAP

※ PTM-Disease Association

NumPTMDiseaseCell TypeTypePTM SitePMID
1PhosphorylationColon cancer/carcinomaA23786838
[Reference]: Expression levels of COX-2 and VEGF protein and phosphorylation of AKT, ERK1/2, and NF-B P65 were also inhibited
2PhosphorylationRecessive Parkinson diseaseA23261939
[Reference]: The?phosphorylation?of Akt and ERK following exposure to H(2)O(2) or MPP(+) was also inhibited in PINK1 P210A mutant cells compared with empty vector-transfected cells.
3PhosphorylationMyelomaP11986233
[Reference]: By Western blot analysis, we demonstrated that the intracellular signaling pathways of IL-21 in myeloma cells involved phosphorylation of Jak1, Stat3, and Erk1/2 (p44/42 mitogen-activated protein kinase).
4PhosphorylationNon-small cell lung cancer/carcinomaP24096476; 23306155
[Reference]: Derlin-1 is overexpressed in non-small cell lung cancer and promotes cancer cell invasion via EGFR-ERK-mediated up-regulation of MMP-2 and MMP-9
5PhosphorylationLung cancer/carcinomaP23149922
[Reference]: Furthermore, MIG-7 protein inhibited protein phosphatase 2A to sustain Akt/GSK3? phosphorylation and cancer-cell migration/invasion
6PhosphorylationColon cancer/carcinomatumor tissueP23183114; 24085800
[Reference]: High nuclear expression of phosphorylated extracellular signal-regulated kinase in tumor cells in colorectal glands is associated with poor outcome in colorectal cancer
7PhosphorylationLiver cancerSK-hep-1 cell lineP23693078
[Reference]: In addition, we observed an effect of ectopic IRTKS expression and PD98059 on HCC proliferation: the result showed that inhibiting ERK phosphorylation could rescue the proliferation-promoting role of IRTKS in the SK-hep-1 and YY-8103 cell lines
8PhosphorylationLymphomacell lineP23620775
[Reference]: In vitro, sorafenib significantly decreased cell proliferation and phosphorylation levels of MAPK and PI3K/Akt pathways while increased apoptotic cell death
9PhosphorylationBladder cancerP24375195
[Reference]: We also examined the level of ERK phosphorylation. We found that CRKL transfection led to the increase of ERK phosphorylation level, without significant change of total ERK.
10PhosphorylationProstate cancer/carcinoma/adenocarcinomaP9927031
[Reference]: We examined 82 primary and metastatic prostate tumor specimens for the presence of activated MAP kinase as a measure of Ras signaling using an antibody that specifically recognizes the dually phosphorylated MAP kinases, ERK1 and ERK2. High levels of activated MAP kinase were observed in high-grade and advanced-stage tumors, suggesting elevated Ras signaling in advanced prostate tumors
11PhosphorylationMelanomaU15208655
[Reference]: BRAF somatic mutations, found in 66% of melanomas, have NIH3T3 transforming ability with the elevated kinase activity in vitro.
12PhosphorylationOvarian cancer/carcinomatumor tissueU23285101; 14961570
[Reference]: In this study, we found that GRB7 (P<0.0001), ERK phosphorylation (P<0.0001) and FOXM1 (P = 0.001) were frequently increased and associated with high-grade tumors, as well as a high tendency in association with advanced stage ovarian cancer by immunohistochemical analysis. Intriguingly, the expressions of GRB7 (P<0.0001),
13PhosphorylationPancreatic cancer/carcinoma/adenocarcinomatumor tissueU24209743; 21678462
[Reference]: Inhibiting ERK1/2 signaling in pancreatic cancer cells decreases pancreatic cancer cell invasion
14PhosphorylationBreast cancer/tumor/carcinomaU24374773; 15193230
[Reference]: The levels of expression and phosphorylation of MEK1/2 and ERK1/2 proteins in four breast cancer cell lines increased notably as compared with those in MCF-10 cells
15PhosphorylationCrohn's diseaseU23970928
[Reference]: The result shows that the phosphorylation of the Ras, Raf-1, MEK-1, and ERK-1/2 proteins and the expression of the corresponding mRNAs in the colon tissue of CD rat were significantly higher than the normal control group.
16PhosphorylationAdenomaU21989899
[Reference]: There was also a statistically significant increase in nuclear staining intensity for pERK (p<0.001) and pMEK (p<0.001) in adenomas compared to non-neoplastic mucosa
17PhosphorylationLung cancer/carcinomaU24286320
[Reference]: We also observed increased extracellular signal-regulated kinase (ERK) phosphorylation and enhanced proliferation of TRAP1 overexpressing cells
18PhosphorylationBipolar disorderU24075327
[Reference]: Western blot and flow cytometry analyses showed increased phosphorylation levels of p65 NF-B subunit, and MAPKs ERK1/2, and p38 in BD patients in euthymia in comparison with controls
19Tyrosine PhosphorylationTuberous sclerosis complexHEK293TUY20417671177
[Reference]: Extracellular signal-regulated kinases (Erk) 1 and 2, the first characterized members of the mitogen-activated protein kinase (MAPK) family, are activated by MAPK kinase (MEK) 1/2??ediated phosphorylation on both Thr202?and Tyr204residues
20Tyrosine PhosphorylationBreast cancer/tumor/carcinomaMCF7 cell lineUY20428415597
[Reference]: Y204 was improved cancer-specifc survival in ductal breast cancer

※ Disease Cross-ref Annotation

DatabaseAnnotation
CTD (Curated)
(count: 27)
(view all)
MESH:D001284 ; Atrophy
MESH:D002545 ; Brain Ischemia
MESH:D002294 ; Carcinoma, Squamous Cell
MESH:D009202 ; Cardiomyopathies
MESH:C566627 ; Ceroid Lipofuscinosis, Neuronal, 6
MESH:D001651 ; Cholestasis, Extrahepatic

※ PTM Sites

PTM Modification Sites
Phosphorylation
(count: 16)
(view all)
130       DLMETDLYKLLKSQQ     dbPAF
170       LHRDLKPSNLLINTT     dbPAF
198       ADPEHDHTGFLTEYV     dbPAF
202       HDHTGFLTEYVATRW     dbPAF
204       HTGFLTEYVATRWYR     dbPAF
207       FLTEYVATRWYRAPE     dbPAF
Acetylation
(count: 3)
220       PEIMLNSKGYTKSID     PLMD
287       YLQSLPSKTKVAWAK     PLMD
294       KTKVAWAKLFPKSDS     PLMD
Ubiquitination
(count: 11)
(view all)
168       NVLHRDLKPSNLLIN     PLMD
181       INTTCDLKICDFGLA     PLMD
220       PEIMLNSKGYTKSID     PLMD
287       YLQSLPSKTKVAWAK     PLMD
289       QSLPSKTKVAWAKLF     PLMD
294       KTKVAWAKLFPKSDS     PLMD
Sumoylation
(count: 1)
357       MELDDLPKERLKELI     PLMD
Malonylation
(count: 1)
220       PEIMLNSKGYTKSID     PLMD
Methylation
(count: 2)
302       LFPKSDSKALDLLDR     PLMD
361       DLPKERLKELIFQET     PLMD

※ Protein-Protein Interaction

NetworkInteraction
ABSource
E2QRG5P27361HPRD
E7EWZ9P27361HPRD
F8VXB4P27361HPRD
H7BXQ4P27361MINT
O14788P27361HPRD
O60504P27361HPRD
O75330P27361HPRD
O75676P27361HPRD; IntAct
O76070P27361HPRD
P00750P27361HPRD
P01106P27361HPRD
P01241P27361IntAct
P02686P27361HPRD
P02741P27361IntAct
P03372P27361HPRD
P04049P27361HPRD
P04629P27361HPRD
P04637P27361HPRD
P05023P27361HPRD
P05412P27361HPRD
P06213P27361HPRD
P06239P27361HPRD
P07101P27361HPRD
P07948P27361HPRD
P07949P27361HPRD
P08047P27361HPRD
P08138P27361HPRD
P08237P27361IntAct
P08575P27361IntAct
P10415P27361HPRD
P14921P27361HPRD
P15923P27361HPRD
P15927P27361BioGRID
P15976P27361HPRD
P16118P27361IntAct
P16949P27361HPRD
P17097P27361HPRD
P17302P27361HPRD
P17480P27361HPRD
P17535P27361HPRD
P17542P27361HPRD
P17600P27361HPRD
P17676P27361HPRD
P18545P27361HPRD
P19235P27361HPRD
P19419P27361HPRD; IntAct
P20338P27361HPRD
P20749P27361HPRD
P23443P27361HPRD
P23467P27361IntAct; MINT
P23469P27361HPRD; MINT
P23470P27361IntAct
P25098P27361HPRD
P26885P27361IntAct
P27361P27361HPRD
P27361P28562HPRD; MINT
P27361P30533HPRD
P27361P31994HPRD
P27361P35236HPRD
P27361P36507HPRD
P27361P36956HPRD
P27361P40763HPRD
P27361P42229HPRD
P27361P43146HPRD
P27361P43405HPRD
P27361P43694HPRD
P27361P45983HPRD
P27361P46734HPRD
P27361P49023HPRD
P27361P49407HPRD
P27361P49815HPRD
P27361P50579HPRD
P27361P51452HPRD; MINT
P27361P51812HPRD
P27361P53004DIP
P27361P53355HPRD; IntAct
P27361P53805HPRD
P27361P54829HPRD
P27361P55211HPRD
P27361P56524HPRD
P27361P57086MINT
P27361P62136MINT;HPRD
P27361P67775HPRD; MINT
P27361P78347HPRD
P27361Q00613HPRD
P27361Q01892HPRD
P27361Q02156HPRD
P27361Q02363IntAct
P27361Q02750HPRD; MINT
P27361Q02880HPRD
P27361Q03135HPRD
P27361Q04828IntAct
P27361Q05469HPRD
P27361Q05513HPRD
P27361Q05655HPRD
P27361Q05923MINT
P27361Q06124HPRD
P27361Q07021HPRD
P27361Q07869HPRD
P27361Q12772HPRD
P27361Q12913IntAct; MINT
P27361Q12967HPRD
P27361Q13115HPRD
P27361Q13177HPRD
P27361Q13480HPRD
P27361Q13501MINT
P27361Q14160IntAct
P27361Q14790HPRD; MINT
P27361Q15121HPRD; MINT
P27361Q15256HPRD; MINT
P27361Q15262IntAct
P27361Q15349HPRD
P27361Q15418HPRD
P27361Q15583HPRD
P27361Q15746HPRD
P27361Q15796HPRD
P27361Q16204HPRD
P27361Q16288HPRD
P27361Q16539MINT;HPRD
P27361Q16559HPRD
P27361Q16665HPRD
P27361Q16690HPRD
P27361Q16827IntAct
P27361Q16828HPRD; MINT
P27361Q6VAB6HPRD
P27361Q8IVT5MINT
P27361Q8IYT8IntAct
P27361Q93045HPRD
P27361Q96SM3IntAct
P27361Q99558HPRD
P27361Q99956HPRD; MINT
P27361Q9BSF8MINT
P27361Q9BUB5HPRD
P27361Q9C0H2IntAct
P27361Q9NQ66HPRD
P27361Q9NZQ3HPRD
P27361Q9P2Y4MINT
P27361Q9UHA4HPRD
P27361Q9UHX1IntAct
P27361Q9UJY1HPRD
P27361Q9UK32MINT
P27361Q9ULV5HPRD
P27361Q9UQC2HPRD; MINT
P27361Q9Y508MINT
P27361Q9Y6W6HPRD